This means it can be viewed across multiple devices, regardless of the underlying operating system. If your scanner saves files as pdf portbale document format files, the potential exists to merge the individual files into one doc. This causes an insulindependent diabetes mellitus called alloxan diabetes in these animals, with characteristics similar to type 1 diabetes in humans. All animals were allowed free access to water and pellet diet. Empirical formula hill notation c 4 h 2 n 2 o 4 h 2 o.
Group 1normal control, nondiabetic rats, 5 standard control, diabetic rats and 6 negative control, diabetic rats were administered. Alloxan induced cuiidependent production of 8oxodg in calf thymus dna in the presence of nadh. I paid for a pro membership specifically to enable this feature. Following injecting rats with alloxan by one hour, rats were allowed to be fed standard pellets and water ad libitum.
Original article rat model of foodinduced nonobesetype 2. Alloxan its toxicologic pathology and homoeopathic. Alloxaninduced diabetes, a common model for evaluating the. A pdf file is a portable document format file, developed by adobe systems. A digital glucometer accuchek advantage, roche diagnostic, germany was used to determine the blood glucose levels of the animals. Optimization for alloxan catalyzed model sulfoxidation with o 2 a entry zh solvent cloading of. Prior to administration of alloxan, mice were fasted overnight but given water ad libitum. Alloxaninduced diabetes, a common model for evaluating. There was no toxicity observed in the aqueous extracts of.
Have eyes examined and tested by medical personnel. A pdf portable document format is a widely popular type of document format created by adobe. Depending on the type of scanner you have, you might only be able to scan one page of a document at a time. Sep 30, 2016 rats selected for the intervention group, were injected with 120 mgkg dose of alloxan monohydrate, 7 days after injecting alloxan monohydrate to confirm the diabetic state in rats, blood glucose level of rats was measured, and rats with blood glucose concentration more than 200 mg100 cm 3 that were diabetics were selected. Hypoglycemic effect of polyherbal formulation in alloxan. The effect of lisinopril on blood glucose level given. Jan 01, 2017 alloxan which is chemically known as 5,5dihydroxyl pyrimidine2,4,6trione is an organic compound, a urea derivative, a carcinogen and cytotoxic glucose analog. Hyperglycemia was recorded in the alloxan and foodinduced groups respectively. Alloxancatalyzed biomimetic oxidations with hydrogen.
Effect of aloe vera leaf extract on alloxan induced diabetes. Effect of aloe vera leaf extract on alloxan induced. Searching for a specific type of document on the internet is sometimes like looking for a needle in a haystack. Effect of ethanolic extract of pterocarpus marsupium root on. Biochemical and haematological effects of the leaf extract. The data presented in table 1 demonstrated that bgl at 100 mgkg b. The anhydrous derivative ocnhco 2 co is also known, as well as a dimeric derivative. Diabetes was induced by injecting it at a dose of 150 mgkg b. Effect of haematogenous oxidation therapy with ul travioletc. Alloxan monohydrate sigma aldrich, germany, was used to cause pharmacological diabetes to confirm the effectiveness of methanol extracts of c. Guidelines on dosage calculation and stock solution. Modulations in antioxidant activities of selected gastro. Biochemical and haematological effects of the leaf extract of.
Administration of rutin prevented urinary ketone body formation and decreased serum creatinine and urea levels in alloxan induced diabetic rats. Alloxan is one of the common diabetogenic agents often used to assess the antidiabetic potential of both pure compounds and plant extracts in studies involving diabetes. All these pdf joiners are completely free and can be downloaded to windows pc. Studies on the administration of vernonia amygdalina del. Adobe systems is a software manufacturer that has created many document and multimedia editing programs.
By michelle rae uy 24 january 2020 knowing how to combine pdf files isnt reserved. Read on to find out just how to combine multiple pdf files on macos and windows 10. The first group control received normal saline and the second group received alloxan monohydrate alone. The rats were injected intraperitoneally with alloxan monohydrate dissolved in sterile normal saline at a dose of 100 mgkg body weight and then again after 2 days, they were administered with alloxan 150 mgkg body weight. Diabetes was induced in 30 albino rat by injecting 150 mgkg of alloxan monohydrate intraperitoneally dissolved in 0. Effects of aqueous extract of alfalfa on hyperglycemia and. Alloxan monohydrate at dose of 100, 140,180 and 200 mgkg b. Glu 28 cose solution 50% was used to prevent the initial hypoglycemia caused by alloxan monohydrate 29.
The animals were divided into 4 groups and were treated with 2 mlkg of normal saline, 100 mgkg, 200 mgkg, 400. Pdf file or convert a pdf file to docx, jpg, or other file format. Hold eyelids apart and flush eyes with plenty of water for at least 15 minutes. Effect of ethanolic extract of pterocarpus marsupium root.
Antidiabetic, haematinic and anticholesterolmic effects. Antihyperglycaemic effect of aqueous and ethanolic. Trigonella foenumgraecum seed and leaf extract on alloxan. Alloxan is an oxygenated pyrimidine and toxic analog of glucose. All other chemicals and drugs used were obtained commercially and were of analytical grade. Louis, mo, usa was used to induce diabetes in adult wistar rats of both sexes. Buy alloxan monohydrate cas 22441, a cytotoxic compound that causes oxidative base damage to nuclear dna, from santa cruz. The hyperglycemia was confirmed after 72 hrs by the elevation of blood glucose and the behavioral changes excess thirst and frequent urination. By inducing dm with alloxan in wistar rats, current study investigated the changes in antioxidant activities of selected gastrointestinal gi tissues stomach, duodenum, pancreas and liver, upon treatment with silymarin andor vitamin c. We also need to disable the popup window which asks us to specify the path in which the downloaded file has to be placed. Three days later, diabetes was confirmed using glucometer one touch, life scan limited, animals were considered diabetic if the blood glucose level was. Antihyperglycaemic effect of aqueous and ethanolic extract. Plant collection the leaves, pulp and bark of adansonia digitata were collected in kadage community of ringim local government area jigawa state nigeria in april 2017. Alloxan monohydrate and other chemicals used in this work were of good grades and were purchased from ekoistic laboratories, port harcourt, nigeria.
Twentyfive 25 albino rats were intraperitoneally administered a freshly prepared solution of alloxan monohydrate 120 mgkg to overnight fasted rats. Alloxan is also used in research models to induce diabetes. Rats with fasting blood glucose more than 220 mgdl was considered for study. Alloxan is a toxic glucose analogue, which selectively destroys insulinproducing cells in the pancreas that is, beta cells when administered to rodents and many other animal species. Alloxan monohydrate used was obtained from sigma chemical company, st.
These pdf mergers let you merge multiple pdf files easily. Research article alloxan induced diabetes mellitus in wistar. The required dose of alloxan monohydrate to induce experimental diabetes intraperitoneally in a rat weighing. Rutin ameliorates metabolic acidosis and fibrosis in.
Join researchers using high quality alloxan monohydrate. Antidiabetic drugs in combination with hydroxychloroquine. Hypoglycaemic activity and nephroprotectective effect of. These pdf merging software offer various features, like. You can merge two pdf files at a time into one pdf file.
Rats were divided into four experimental groups n4 receiving different treatments. Pdf is a hugely popular format for documents simply because it is independent of the hardware or application used to create that file. After that, the experimental rats were administered 20%. Diabetes was confirmed by testing blood glucose level using a. By richard morochove, pcworld practical it insight from tony bradley todays best tech deals picked by pcworlds editors top deals. After 24 hours, blood glucose content was measured by using clever check glucose test meter bio land, germany using blood sample collected from the tail vein of the rats. If your pdf reader is displaying an error instead of opening a pdf file, chances are that the file is c. The new yahoopowered ads for adobe pdf service makes it easy to place payperclick ads in your pdf files. Select the first and second pdf file to be merged and name the final file and location, click convert button and the software will create a merged file for you. Catalase and bathocuproine, a cuispecific chelator, almost completely inhibited dna damage, suggesting the involvement of h2o2 and cui.
To merge more files follow the same process, your first file in second process will be the output file of first process, and so on. Alloxan catalyst 5c, as its monohydrate form, was purchased from commercial suppliers. A 10 ml blood was collected from diabetic rabbits, blood was being perfused with oxygen for 10 seconds and uvc was irradiated to the blood, uvirradiated. Animals were allowed to fast for 24 hours prior to the injection of freshly prepared alloxan monohydrate 150mgkg, ip 7dissolved in saline solution. Toxicity was observed in the rats using alloxan monohydrate at a. The pathophysiology and histopathology of dm were studied.
To download the pdf file from a webpage, we need to specify the locator to identify the link to download. The effect of lisinopril on blood glucose level given alone. The rats with blood glucose level more than 250 mgdl were used for the study. Rutin ameliorates metabolic acidosis and fibrosis in alloxan.
Alloxan induced diabetic model alloxan monohydrate was. After 72 h of alloxan monohydrate injection, the diabetic. Screening for antidiabetic activity of the ethanolic. The effect of abelmoschus esculentus in alloxaninduced. Screening for antidiabetic activity of the ethanolic extract of. The compound has the molecular formulae, c 4 h 2 n 2 o 4 and a relative molecular mass of 142. Research article alloxan induced diabetes mellitus in. Diabetes was confirmed by determining the blood glucose concentration after 48 hours of alloxan injection. A freshly prepared alloxan monohydrate 65 mgkg in 0. Blood samples collected before the administration of alloxan and after 4 days of alloxan administration.
After that, the experimental rats were administered 20% glucose solution for 24 hours to prevent hypoglycemia. All animals were allowed free access to water and pellet diet and maintained at room temperature in plastic. After 72 hr mice with blood glucose level above 200 mgdl were considered to be diabetic and were used for experimentation. The anhydrous derivative is also known as a dimeric derivative. Treatment groups 2, 3 and 4 had diabetic rats administered 100, 250 and 350 mgkg of combined extracts respectively. Alloxan monohydrate and all the other chemicals were acquired from s.
Whether after 10 to 60 min incubation of pieces of pancreas in the presence of 214c alloxan or 60 min to 24 h after intravenous injection of 214c alloxan to control and insulintreated stz rats, the radioactive. This study aimed to create diabetic animal models and to provide an exact and detailed account of alloxan induced diabetes in rabbits. Phytochemicals, hypoglycemic and hypolipidemic effects of. Pdf a raman spectroscopic investigation of alloxan and. Alloxan induced dna cleavage frequently at thymine and cytosine residues in the presence of nadh and cuii. These are some of the earliest known organic compounds. Luckily, there are lots of free and paid tools that can compress a pdf file in just a few easy steps. A single intraperitoneal administration of alloxan monohydrate 150 mgkg body weight was used to induce diabetes in wistar rats. All other chemicals used for this study were of analytical grade. Alloxan monohydrate is a hydrated form of alloxan, which has been shown to destroy. Alloxan, diabetes mellitus, toxicologic pathology, degeneration, bcells.
Alloxan monohydrate solution was administered at a single dose of 150 mgkg body weight intraperitoneally. Weight of experimental rats before and after induction of alloxan monohydrate. Sometimes before starting off with selenium testing pdf files, we need to download them. For experimental induction of diabetes alloxan monohydrate a743 sigma aldrich was used.
Type 1 diabetes and hyperlipidemia were induced by intravenous injection of alloxan monohydrate and subcutaneous injection of poloxamer 407, respectively. Animals were fasted for 18 h before the experiment and divided into 5 groups 6 animals in each group. Optimization for alloxan catalyzed model sulfoxidation with o 2 table s1. Alloxan monohydrate c4h4n2o5 cid structure, chemical names, physical and chemical properties, classification, patents, literature, biological. Two days after alloxan injection, rats screened for diabetes having glycosuria and hypoglycaemia with blood glucose level of 200 260 mg100 ml were taken for the study. Non hazardous chemical spreadsheet auburn university. Select multiple pdf files and merge them in seconds. Since alloxan is capable of producing fatal hypoglycemia as a result of massive pancreatic insulin release, rats were treated with 20% glucose solution orally after 6 h. First aid measures description of first aid measures. Alloxan monohydrate is a common drug used for developing experimental diabetes in animals, as streptozotocin, though less toxic than alloxan is considerably costlier. This article explains what pdfs are, how to open one, all the different ways. Phytochemical screening and evaluation of nephroprotective. Diabetes was induced using alloxan monohydrate 100 mgkg. Group iv rats were injected with a single dose of alloxan monohydrate 150 mgkg bodyweight and administered rutin hydrate 100 mgkg for a period of 4 weeks by oral gavage.
Antidiabetic, haematinic and anticholesterolmic effects of. Alloxan is a member of the class pyrimidone, with a structure similar to that of perhydropyrimidine. After four days of alloxan induction, animals with blood glucose level 250mgdl were chosen for further studies. Alloxan monohydrate was purchased from sigma chemicals st. Only alloxanised hyperglycaemic animals were used for further studies. Original article rat model of foodinduced nonobesetype.
Antihyperglycemic effect of gasca d herbal formulation on. Diabetes was induced in 16 hrs fasted albino rats with single intraperitoneal dose of alloxan monohydrate. Molecular docking and preclinical studies of gymnema. Rats were divided into five groups of six rats each. The uptake of 214c alloxan by the pancreatic gland was investigated in control and streptozotocininduced diabetic stz rats, using both in vitro and in vivo techniques. Alloxan or alloxan hydrate is an organic compound with formula c 4 h 2 n 2 o 4. Alloxan its toxicologic pathology and homoeopathic utility. The hydrogen bonding network in the crystal of the so called alloxan monohydrate, although. Alloxan, sometimes referred to as alloxan hydrate, is the name of the organic compound with the formula ocnhco 2 coh 2. To combine pdf files into a single pdf document is easier than it looks. Most electronic documents such as software manuals, hardware manuals and ebooks come in the pdf portable document format file format.
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